Composite

Part:BBa_K3187013:Design

Designed by: iGEM TU_Darmstadt 2019   Group: iGEM19_TU_Darmstadt   (2019-10-13)


P22 Coat and Scaffolding Protein x Coat Protein with LPETGG-tag for sortase-mediated ligation


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 76
    Illegal EcoRI site found at 1395
    Illegal XbaI site found at 3505
    Illegal PstI site found at 1931
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 76
    Illegal EcoRI site found at 1395
    Illegal NheI site found at 1936
    Illegal PstI site found at 1931
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 76
    Illegal EcoRI site found at 1395
    Illegal BamHI site found at 1389
    Illegal XhoI site found at 2104
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 76
    Illegal EcoRI site found at 1395
    Illegal XbaI site found at 3505
    Illegal PstI site found at 1931
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 76
    Illegal EcoRI site found at 1395
    Illegal XbaI site found at 3505
    Illegal PstI site found at 1931
    Illegal NgoMIV site found at 1783
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Ordered from Integrated DNA Technologies (IDT) and cloned in pTetW3con2

References